Toward chromosome 4, the three QTLs have been in the 66
Box plots appearing the consequences of your own marker genotypic classes (AAAA, AAAB, AABB, ABBB, BBBB) out of cuatro applicant SNPs for the tuber sugar concentration (GLU); bud-stop fry colour (FBE); stem-tuber fry color (FSE); ID, Idaho
The new co-localization off multiple QTLs within maturity locus area in this analysis is similar to the noticed models out of phenotypic correlations. 01) with lots of attributes overlapping an equivalent genetic condition Palm Bay escort (Fig. 1). Such as, EBID11 and you can VWID11 score one another displayed a strong bad correlation with MAT95ID and you will MAT120ID. Which trend get give an explanation for big perception QTL with later maturity closely associated with the delay start of VW and you can EB, or alternatively, very early readiness ultimately causing early start of VW and EB. A comparatively higher relationship has also been seen between vine readiness and you may increases routine, that have early readiness in the significantly more spread growth habit. On the other hand, a decreased relationship (roentgen ? 0.2) anywhere between vine maturity and each other tuber sugar focus and you can bud-prevent fry colour was in keeping with different types to your QTL outcomes.
Tuber glucose, bud-prevent and stalk-stop fry colour
In addition to chromosome 5, significant QTLs to have sugar, bud-stop and you can stem-avoid fry color was in fact perceived inside the overlapping areas of chromosomes 4, 6, 10, and you can eleven. 0 cM to your LOD scores between cuatro.5 to 6.0 (Table step three, Fig. 4). Such QTLs said ranging from seven.dos and you can eleven.3% of the attribute variance. Studies various effortless activities shown a single ideal-fitting model for all around three qualities, with a two fold-simplex allele (AAAB ? AAAB) to your homologous chromosomes H2 and H5 off RGR and you will Public relations, respectively (Desk 3, Most document 8: Contour S4). To own sugar, the fresh new B allele try regarding the large glucose concentration, when you’re to possess bud-avoid and you can stalk-end fry colour, the current presence of new B allele expressed down reflectance viewpoints (Fig. 3b).
LOD get shipments from QTLs for a few traits over the a dozen potato chromosomes (Chr). Abbreviations try as in Fig. step 1
A candidate SNP for a double-simplex configuration on H2 and H5 is solcap_c2_12976 at position 67.4 cM. On the reference PGSC v4.03 Pseudomolecules, this SNP maps to the long arm of chromosome 4 at position 69.1 Mb on the F-box and WD40 domain protein gene (PGSC0003DMG400003339), which is close (< 2 Mb) to several genes associated with starch/sugar inter-conversion as annotated in the potato reference genome, and/or associated with tuber quality traits . They include alpha amylase (AMY-4/1 PGSC0003DMG400007974, AMY-4/2 PGSC0003DMG400009891), hexose transporter (HT-4/3, PGSC0003DMG400009994), invertase (NV-4, PGSC0003DMG400009936), and starch branching enzyme I (SBE I, PGSC0003DMG400009981).
On chromosome 6, significant QTLs mapped at 53 cM for glucose and at 43 cM for FBE and SBE, respectively. The LOD profiles ranged from 5.4 to 7.6 and explained between 10.8 and 16.1% of the trait variance, respectively (Table 3, Additional file 9: Figure S5). The best model for all three traits was a double-simplex configuration with allele effects on H2 and H5. As in chromosome 4, the presence of the B alleles was associated with an increase in glucose concentration and a decrease in reflectance readings (Fig. 3c). There are four candidate SNPs with a double simplex configuration nearby (solcap_snp_c2_33777, solcap_snp_c1_10130, solcap_snp_c2_5769, solcap_snp_c2_56145, c1_3003). All were significantly (P-value < 0.0001) associated with the mean trait values, however, solcap_snp_c2_56145 had on average the highest percent of variance explained (9% to 16%). The SNP solcap_snp_c2_56145 maps at position 51.4 Mb of the potato reference genome on the ATP binding protein gene PGSC0003DMG400033089. This SNP is located in a region (
eight Mb) that has multiple family genes associated with the starch/glucose inter-conversion including hexokinase (HXK-six, PGSC0003DMG400016521), fructokinase (FRK-6/step one, PGSC0003DMG400027017, FRK-6/2, PGSC0003DMG400026916), and you will invertase (INV-na-6/step one, PGSC0003DMG400026107, INV-6/dos, PGSC0003DMG400033142) family genes.